Biotek EL800, Operator'S Manual

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Define (Method, Map, Formula and Curve)
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ELx800 Operator’s Manual
Defining FORMULA
The ELx800™ supports three types of formulas (Cutoff, Transformation, and
Validation), as well as the ability to program variables for use within formulas. Up
to three types of Validation formulas may be defined (Blank, Control, and Assay
Validation).
Formula definition is not available in the EL800 version.
To define formulas:
1. Start at the Main Menu and select
DEFINE .
2. Select the assay then press ENTER to display the DEFINE options screen.
D E F I N E :
M E T H O D M A P F O R M U L A C U R V E
3. Select
FORMULA
. The
SELECT FORMULA TYPE
screen will appear
(described on the next page).
Calculation Structure
During data reduction, formulas are processed in the order shown below. The
number of permitted formulas of each type is shown as well.
•
Blank Validation 0-1
•
Control Validation 0-4
•
Assay Validation 0-4
•
Transformations 0-1
•
Cutoff Formulas 0-1
•
Curve-Fit Analysis (if a curve-fit method is defined)
To capture and manipulate the raw data using 384-well
microplates with the ELx800NB, you must use Gen5™, KC4™,
or KCjunior™. Gen5, KC4, and KCjunior are PC-based software
programs you can use to set up your assay, communicate with
the ELx800 to run the assay, and then manipulate the raw data
that is automatically retrieved from the reader. Refer to
Appendix B
for additional information.