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Chapter 6: Troubleshooting and Error Codes
BioTek Instruments, Inc.
Overview
If an error code appears on the display (or in Gen5™, KC4™, or KCjunior™), consult
the error code list to see if the error is easily correctable. If you cannot resolve the
problem, run the instrument
System Test
and note the exact error code and serial
number of the reader. Then call BioTek’s Technical Assistance Center (refer to
Chapter 1
for contact information).
System Test Description
The System Test feature conducts a series of tests, at each of five set wavelengths,
which confirm adequate light levels, low electronic noise, adequate photodiode
sensitivity, and overall system cleanliness. The testing is designed to ensure that the
ELx800™ will give in-specification performance for each set wavelength over the
specified OD range.
The reader automatically runs an internal System Test each time it is powered on. The
reader will “chirp” repeatedly if the power-on System Test results do not meet the
internally coded Failure Mode Effects Analysis (FMEA) criteria established by BioTek.
A system test should then be initiated to try to retrieve an error code from the reader.
Glossary of Terms
•
Air Blank:
A full light reading through a filter with no plate in the light
path.
•
Dark Current:
A reading taken with the light blocked to measure
background light levels in the reading chamber. Also used as a measure of
background electronic noise within the measurement circuit.
•
Gain:
An automatic electronic adjustment to the measurement circuit. The
gain adjustment compensates for changing light levels or filter variations.
For example, if the lamp output decreases slightly, the gain will increase to
make up the difference.
•
Axis:
Refers to a motor for the filter wheel or plate carrier.
•
Offset:
A numerical limit, usually a range. For example, if the gain fails an
offset test, it may be too high or too low.
Summary of Contents for EL800
Page 1: ...Operator s Manual Absorbance Microplate Reader ELx800 ...
Page 2: ... ...
Page 4: ... ...
Page 62: ...36 Chapter 2 Installation BioTek Instruments Inc ...
Page 126: ...100 Chapter 3 Operation BioTek Instruments Inc ...
Page 162: ...136 Chapter 5 Maintenance and Decontamination BioTek Instruments Inc ...
Page 182: ...156 Appendix A Computer Control BioTek Instruments Inc ...
Page 194: ...168 Appendix B Using 384 Well Geometry BioTek Instruments Inc ...
Page 197: ... 171 ELx800 Operator s Manual Figure 39 Curve Fit Report ...
Page 199: ... 173 ELx800 Operator s Manual Figure 41 Column Report without samples ...
Page 200: ...174 Appendix C Report Format BioTek Instruments Inc Figure 42 Panel Report ...
Page 201: ... 175 ELx800 Operator s Manual Figure 43 Assay Detail Report Sheet 1 of 2 ...
Page 204: ...178 Appendix D Comparison of the ELx800 and the EL800 BioTek Instruments Inc ...
Page 218: ...192 Appendix E Instructions for Programming a New Assay BioTek Instruments Inc ...