Biotek EL800, Operator'S Manual

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Controlling the Reader With Gen5™
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ELx800 Operator's Manual
To perform a “Quick Read”:
1. At Gen5’s Welcome screen, select System Menu|File|New Experiment.
(Alternative: select Read a Plate at the Welcome screen, then proceed to
step 4 below.)
2. Click Default Protocol , then click OK . Gen5 will open the Experiment
workspace, which includes the Protocol menu tree and Plate screen.
3. Select Plate|Read or click the ‘Read Plate’ icon. The ‘Procedure’ dialog
will open.
Gen5 and Gen5 Secure: If more than one reader was added in Gen5, the
‘Instrument Selection’ dialog will appear instead of the Procedure
dialog. Select
ELx800
, then click
OK
. The Procedure dialog will then
appear.
4. Select a Plate Type .
5. Click Read to open the ‘Read Step’ dialog.
6. Select a Read Type .
7. Define the wavelength(s) at which the plate will be read.
8. Define other reading parameters as desired. Click the Help button for
assistance.
9. When complete, click OK to return to the Procedure dialog.
Click
Validate
if you would like Gen5 to verify the defined parameters.
If all parameters are valid, you will receive confirmation. If any
parameters are invalid, Gen5 will provide information for correcting the
problem. Refer also to the Troubleshooting section of the Help system.
10. Click OK again to save and close the Procedure dialog. The ‘Plate Reading’
dialog will open.
11. Enter any desired information, place the plate on the carrier, then click
READ to begin the plate read. Click OK when the ‘Load Plate’ dialog
appears. The plate will be read.
To view the raw data results, use the Data drop-down arrow in the Plate
screen to select one wavelength. The results will be displayed for the
selected wavelength. Repeat, for other wavelengths.
To analyze, manipulate, or print results, Protocol parameters should be
defined. Refer to Gen5’s Help system for instructions.
Gen5 Reader Control does not support data reduction.