Biotek EL800, Operator'S Manual

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Chapter 3: Operation
BioTek Instruments, Inc.
Reuse of Standard Curves
The ELx800™ has the ability to reuse a standard curve that has already been
established.
Limitations of the Reuse of Standard Curves
Standard curves can only be reused in assay positions 31 through
55. Each of these positions can only store one standard curve.
Standard curves cannot be reused on panels (see page 83 for Panel
Definition).
Standard curves will be stored in memory with the Assay Name,
Standard Concentrations, Replicate Counts, and Optical Density
values
for each standard replicate.
Stored standard curves can only be reused for the assay that the
curve was originally generated on (e.g., the curve for Assay 53
cannot be applied to samples on a plate to be run in Assay 51).
To reuse a standard curve, you must first program an assay (in
positions 31 through 55) and then run the assay. During the defining
process, you will be prompted to enter the number of standards, the
number of standard replicates, and the standard concentrations
. The
following screen will appear after these prompts:
R E U S E S T A N D A R D C U R V E ? Y E S
Y E S N O
After the assay has been run, the results have been calculated, and the reports
have been generated, the reader will prompt if this standard curve should be
stored in memory. The following display will appear:
S A V E S T A N D A R D C U R V E ? Y E S
Y E S N O
Select
YES
to store the curve for use at a later time. The next time a plate is to
be read using this assay, the instrument will prompt if there are standards on
the plate. Select
NO
to discard the curve.