Chip View updates to show the lanes to be used in the run as green (left). Lane
usage is calculated based on the number of samples, assay type, primer pools
used, and minimum reads per sample entered at assay setup.
If the minimum reads per sample × the number of samples exceeds the chip or
lane well capacity, a dialog box appears after you click Assign asking you to
confirm that you want to continue. After confirmation, the Chip View updates
and shows the lane color as red instead of green. In the example shown at right,
seven samples were selected instead of four. The run is allowed, but you may not
achieve the required reads per sample to pass QC metrics.
5.
If you selected more than one assay, repeat step 4 for each additional assay.
6.
If needed, edit samples in one of the following ways, then click Next.
• Click View and Remove, make your selections, then click Update.
• Click Remove All, make your selections, then click Assign.
Chapter 5
Create and manage run plans
Create a run plan for a sample run
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Genexus
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Integrated Sequencer User Guide
