Field Service Engineers view and sign off on verification run results.
1.
In the menu bar, click Results
4
Verification Results
2.
In the Results > Verifications Results screen, click View Results in the Actions
column for a verification run to view verification results.
3.
In the Verification Result screen, click Sign Off to open the Electronic
Signature dialog box, then enter your user name, password, and optional
comments.
4.
In Footer Field, enter any text that you want to appear in the footer of the PDF
report pages.
5.
Click Sign Off to confirm your electronic signature.
System Install Qualification results
For a System Install Qualification run, basic information about the verification run is
provided, and the pass/fail QC status for the following metrics are reported for the
controls that are used in the run:
QC Metric
Description
Run QC
AQ20 Mean Read Length
Average read length, in base pairs, at which the error rate is ≤1% for all
aligned reads.
Raw Read Accuracy
The percentage of raw reads mapped to the reference genome.
Run Balance Factor
The minimum Sample Balance Factor in a set of four tested samples A–
D.
Total Reads
The total number of filtered and trimmed reads with the listed barcodes
assigned to the samples.
Templating Control QC – CF-1
Average Reads Per Lane
Average number of CF-1 reads per GX5
™
Chip lane.
Base Call Accuracy
1 – (total number of errors for all positions in the control / total number
of aligned bases).
Mean AQ20 Read Length (bp)
Average length, in base pairs, at which the error rate is ≤1% for all
aligned reads of the CF-1 control fragment.
Library QC – DNA
Amplicons With No Strand Bias
The percentage of amplicons with a strand bias between 30% and 70%.
AQ20 Mean Read Length
Average read length, in base pairs, at which the error rate is ≤1% for all
aligned reads.
Percent Reads On Target
The percentage of reads mapped on target to total reads.
View and sign off
on the verification
run results (Field
Service Engineer)
Chapter 9
Review data and results
Verification runs
9
114
Genexus
™
Integrated Sequencer User Guide
