TriContinent C24000, Product Manual

Page: 45 / 144

Priming syringe and tubing
45
C-Series Product Manual
Priming syringe and tubing
Priming prepares the system for operation by removing air from the syringe
and tubing.
1.
Run the pump with a full stroke and several cycles to prime the system with
the working fluid (e.g. water). The following example is in DT protocol.
Example priming string:
[ZV6000gIA3000OA0G3R]
Initialize [Z]; set priming speed [V6000]; set start of looping sequence
[g]; move valve to inlet position and fill, with plunger drawing down to
maximum position A3000 [IA3000]; move valve to outlet and dispense,
with plunger pushing up to minimum position A0 [OA0]; repeat 3 times
[G3].
–
The number of cycles depends on the syringe size and internal volumes
of the inlet and outlet tubing connected to the pump.
–
Smaller syringes can be harder to prime due to lower fluid velocity, and
require more strokes.
2.
Observe for bubbles. If any are observed, run the priming sequence again,
perhaps specifying more cycles with the [G] command. Check all connec-
tions of tubing and syringe for leaks.
Surfactants can be added to the working fluid to reduce surface tension
and help flush bubbles out of the system. The smallest syringes (50
µ
L),
for example, have been primed with alcohol followed by distilled water
to help remove air bubbles.
Once priming has been completed, the pump is ready for comprehensive
programming.