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5
Protein Applications
Measure Protein A280
Thermo Scientific
NanoDrop Eight User Guide 55
Reported Values
•
Protein concentration.
Reported in selected unit
(mg/mL or µg/mL). Calculations are based on
Beer-Lambert equation using corrected protein
absorbance value.
•
A260/A280 purity ratio
. Ratio of corrected
absorbance at 260 nm to corrected absorbance at
280 nm. An A260/A280 purity ratio of ~0.57 is generally
accepted as “pure” for proteins.
Note
: Although purity ratios are important indicators of
sample quality, the best indicator of protein quality is
functionality in the downstream application of interest (e.g.,
real-time PCR).
•
Contaminant
- Displays contaminant identified by the
Acclaro software, if one is available.
•
Corrected
- Display the corrected analyte concentration
determined using the Acclaro software, if one is available.
Summary of Contents for NanoDrop Eight
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