8
Maintenance
Instrument Diagnostics
140 NanoDrop Eight User Guide
Thermo Scientific
8. Ensure PV-1 solution is thoroughly mixed by vigorously shaking both ampoules.
Allow the solution to collect in the bottom portion of the ampoule, if needed
gently tap the ampoule.
9. Carefully snap off top portion of each ampoule using an ampoule cracker,
discard top along with ampoule cracker (use proper safety precautions for
disposal).
10. Use the provided transfer pipette to dispense the contents of both PV-1
ampoules into each well of the 8-well PCR strip tube.
11. Using an 8-channel pipettor, withdraw 1.5 µL of PV-1 solution from each of the
8- wells of the PCR strip tube and pipette onto each of the 8 lower pedestals.
Lower arm.
12. After the measurement is complete, remove sample from both upper and lower
pedestals using a dry laboratory wipe.
13. Repeat steps 11 and 12 to measure five additional individual replicates of the
PV-1 solution.
a. Always use fresh pipette tips to remove each aliquot and use a fresh aliquot
of PV-1 for each measurement.
b. In between each measurement, remove PV-1 solution from all 16 pedestals,
upper and lower, using a clean, dry laboratory wipe.
14. After each measurement is complete, the individual results will be displayed on
screen and subsequently added to the existing results.
15. After six replicates have been measured, the
Status
columns for each
pathlength will indicate whether the channel passed the Performance
Verification.
To interpret performance verification results
1. Results will display
for Pass,
for Conditional Pass, and
for Fail.
a. If results are not within specifications, repeat the procedure using 2 µL
aliquots of PV-1.
b. If results fail to meet specifications using 2 µL aliquots, contact support or
local distributor for assistance.
2. Click
End Experiment
when done.
a. Results can be exported and printed at this time or later from the History.
b. The Experiment name can be changed at this time and Identifiers can be
added.
Note
If Auto-Measure feature is
OFF
, click Measure to begin the
measurement. If Auto-Measure feature is
ON
, the measurement will begin
automatically after the arm is lowered.
Summary of Contents for NanoDrop Eight
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