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7
Learning Center
Acclaro Sample Intelligence
120 NanoDrop Eight User Guide
Thermo Scientific
1
Based on total sample absorbance (sample plus contaminant)
2
Based on corrected sample absorbance (sample minus contaminant)
Since phenols absorb light near the analysis wavelengths for nucleic acid (230 nm,
260 nm, and 280 nm), the presence of phenol in the nucleic acid sample shown
above has pushed the A260/A280 and A260/A230 ratios out of range and caused
the reported nucleic acid concentration to be higher than the real value. The
software identifies the impurity (phenol), and reports the following:
• baseline-corrected absorbance due to phenol (2.53) at the analysis wavelength
(260 nm)
• % coefficient of variation for the measurement result (uncertainty x
100/measurement result = 4.22%; a high %CV indicates the measurement result
is close to the instrument detection limit or there is an interfering component)
• original nucleic acid concentration (462.6 ng/µL), which is based on the total
baseline-corrected absorbance (sample plus contaminant) at the analysis
wavelength
• corrected nucleic acid concentration (286.4 ng/µL), which is based on the
corrected absorbance (sample minus contaminant) at the analysis wavelength
Phenol spectrum
Original nucleic acid spectrum
Corrected nucleic acid spectrum
Out-of-range
purity ratios
Original nucleic acid
concentration
1
Corrected nucleic acid concentration
2
Phenol absorbance
value at 260 nm
% coefficient of variation
Summary of Contents for NanoDrop Eight
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Page 56: ...5 Protein Applications Measure Protein A280 56 NanoDrop Eight User Guide Thermo Scientific...
Page 76: ...6 Custom Applications Measure Custom 76 NanoDrop Eight User Guide Thermo Scientific...
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