ThermoFisher Scientific NanoDrop Eight, User Manual

Page: 48 / 152

5 Protein Applications
Measure Protein A280
48 NanoDrop Eight User Guide Thermo Scientific
Other protein
(

+ MW)
User entered molar
extinction coefficient
and molecular
weight Assumes protein has known molar extinction
coefficient (

) and molecular weight (MW),
where:
(

molar
)*10=(

percent
)*(MW
protein
)
Enter MW in kiloDaltons (kDa) and molar
extinction coefficient (

) in M
-1
cm
-1
divided by
1000 (i.e.,

/1000). For example, for protein
with molar extinction coefficient of 210,000
M
-1
cm
-1
, enter 210.
Other protein
(

1%)
User entered mass
extinction coefficient Assumes protein has known mass extinction
coefficient (

). Enter mass extinction
coefficient in L/gm-cm for 10 mg/mL (

1%)
protein solution.
a
To add or edit a custom protein, use Protein Editor.
Setting Available Options
Mass Ext.
Coefficient
(L/gm-cm)
Description
Setting Available Options
Mass Ext.
Coefficient
(L/gm-cm)
Description
Baseline
Correction On or off
Enter baseline
correction
wavelength in nm or
use default value
(340 nm)
N/A Corrects for any offset caused by light
scattering particulates by subtracting
measured absorbance at specified baseline
correction wavelength from absorbance
values at all wavelengths in sample spectrum.
As a result, absorbance of sample spectrum is
zero at specified baseline correction
wavelength.
Tip : If the sample has a modification that
absorbs light at 340 nm, select a different
correction wavelength or turn off Baseline
Correction.