5
Protein Applications
Measure Protein A280
42 NanoDrop Eight User Guide
Thermo Scientific
Before you begin...
Before taking pedestal measurements with the NanoDrop Eight instrument, lift the
instrument arm and clean the upper and lower pedestals. At a minimum, wipe the
pedestals with a new laboratory wipe. For more information, see
To measure a Protein A280 sample
1. From the Home screen, select the
Proteins
tab and select
Protein A280
.
2. Specify a
if desired.
3. Pipette 1–2 µL blanking solution onto the lower pedestal and lower the arm.
4. Click
Blank
and wait for the measurement to complete.
Tip
: If
is On, the blank measurement starts automatically after you
lower the arm.
5. Lift the arm and clean all pedestals with a new laboratory wipe.
6. Pipette 2 µL sample solution onto the pedestal and lower the arm.
7. Start the sample measurement:
–
–
if Auto-Measure is off, lower arm and click
Measure
.
When the sample measurement is completed, the spectrum and reported values
are displayed (see the next section).
8. When you are finished measuring samples, click
End Experiment
.
9. Lift the arm and clean all pedestals with a new wipe.
Summary of Contents for NanoDrop Eight
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