6
Custom Applications
Measure UV-Vis
62 NanoDrop Eight User Guide
Thermo Scientific
Settings for UV-Vis Measurements
To show the UV-Vis settings, from the Home screen, from the
Custom
tab, select
UV-Vis
.
Setting
Available Options
Description
Monitored
wavelengths
Enter up to 40
wavelengths between
190 nm and 850 nm
User-defined wavelengths to be measured and
reported at run time
. Absorbance values for the first
three entered wavelengths are displayed in the
screen. To see absorbance values for 8
monitored wavelengths, swipe left in the measurement
screen to show the
. To see all monitored
wavelengths, press and hold a sample row to show the
screen (scroll up to display absorbance
values for any additional user-defined wavelengths).
Note
: If Baseline Correction is selected, all displayed
absorbance values are the corrected values.
Analytical
Wavelength
Any wavelength between
190 nm and 850 nm
This is the wavelength the software will use to determine
the pathlength selection.
Automated
Pathlength
On or Off
(affects pedestal
measurements only)
Optional automated pathlength selection
. Allows
the software to use the optimal (shorter) pedestal
pathlength for high concentration samples to help prevent
detector saturation (see
for details).
• When selected, the shorter pathlength is used when
any wavelength has 10 mm equivalent absorbance
value of 12.5 or higher.
• When deselected, the pedestal pathlength is restricted
to 1.0 mm across all wavelengths.
Note
: In either case, displayed absorbance values have
been normalized to a 10 mm pathlength equivalent.
Baseline
Correction
On or off
Enter baseline correction
wavelength in nm or use
default value (750 nm)
Optional user-defined baseline correction
. Can
be used to correct for any offset caused by light scattering
particulates by subtracting measured absorbance at
specified baseline correction wavelength from absorbance
values at all wavelengths in sample spectrum. As a result,
absorbance of sample spectrum is zero at specified
baseline correction wavelength.
Summary of Contents for NanoDrop Eight
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