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Thermo Scientific
NanoDrop Eight User Guide 41
5
Protein Applications
Measure Protein A280
Measure Protein Concentration at A280
Use the Protein A280 application to quantify purified protein samples that contain
amino acids such as tryptophan or tyrosine, or cys-cys disulfide bonds, which
exhibit absorbance at 280 nm. This application reports protein concentration
measured at 280 nm and one absorbance ratio (A260/A280). A single-point baseline
correction can also be used. This application does not require a standard curve.
To measure Protein A280 samples
Measures the concentration of purified
protein samples that absorb at 280 nm.
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will
flow into the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will
permanently damage the quartz fiber optic cables.
Summary of Contents for NanoDrop Eight
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