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5
Protein Applications
Measure Protein A280
44 NanoDrop Eight User Guide
Thermo Scientific
• Choosing a blank:
–
For the Protein A280 application, blank with the same buffer solution used to
resuspend the analyte of interest. The blanking solution should be a similar
pH and ionic strength as the analyte solution.
• Run a
to assess the absorbance contribution of your buffer
solution. If the buffer exhibits strong absorbance at or near the analysis
wavelength (typically 280 nm or 205 nm), you may need to choose a different
buffer or application. See
Choosing and Measuring a Blank
for more information.
• For micro-volume measurements:
–
Ensure pedestal surfaces are properly
and
(Proteins
tend to stick to pedestal surfaces.)
–
Gently (but thoroughly) vortex samples before taking a measurement. Avoid
introducing bubbles when mixing and pipetting.
–
Follow
best practices for micro-volume measurements.
–
Use a 2 µL sample volume. See
information.
Related Topics
• Best practices for protein measurements
• Measure a Micro-Volume Sample
Note
Buffers such as Triton X, RIPA, and NDSB contribute significant
absorbance and are not compatible with direct A280 or A205
measurements.
Summary of Contents for NanoDrop Eight
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