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8
Maintenance
Instrument Diagnostics
Thermo Scientific
NanoDrop Eight User Guide 139
Sample Loading Hints
• Ensure the NanoDrop Eight instrument is not situated near an air vent or an
exhaust fan from a nearby instrument.
• Use a small-volume (0.5 - 10 µL) 8-channel pipettor to load the diH
2
0 and the
PV-1 aliquots.
Discharge and Touch Sample Delivery Method Practice
It is very important to deliver the PV-1 aliquots to all eight pedestals in a single
motion using a multichannel pipettor. It is highly recommended that the steps below
be practiced using water before starting the calibration check procedure.
1. When the pipette tips are close to the measurement pedestals, discharge the
fluid and allow the drops to hang on the end of the tips.
2. Gently touch the droplets to the pedestals and allow them to be pulled off the
tips and onto the pedestals by surface tension.
Tip:
It is important to use tips with a rigid structure to ensure the tips do not
splay or skew when delivering the samples.
Tip:
It is suggested that this technique be practiced until consistent delivery to all
eight positions is routine and quick.
To run performance verification
1. Click on
Performance
at the bottom of the screen.
2. Enter Target Absorbance Value #1 (found on PV-1 ampoule label) into
corresponding
Target #1 Abs
entry box.
3. Repeat step 2 for the
Target #2 Abs
entry box, using Target Absorbance
Value #2 found on PV-1 ampoule label.
4. Once the target values have been entered, click
Continue
.
5. Add 55 µL of diH
2
0 to each well of one of the 8-well strips provided. Do
NOT
aliquot the PV-1 at this time.
6. Use an 8-channel pipettor to pipette a 1.5 ìL aliquot of diH2O onto each of the
lower pedestals, lower the arm, and click
Blank
.
Tip:
It is important to use tips with a rigid structure to ensure the tips do not
splay or skew when delivering the samples.
7. Remove water from upper and lower pedestal surfaces using a clean, dry
laboratory wipe.
Note
Target Abs. values are lot specific and must be entered into the
correct, corresponding entry box.
Summary of Contents for NanoDrop Eight
Page 6: ...Contents vi NanoDrop Eight User Guide Thermo Scientific...
Page 24: ......
Page 56: ...5 Protein Applications Measure Protein A280 56 NanoDrop Eight User Guide Thermo Scientific...
Page 76: ...6 Custom Applications Measure Custom 76 NanoDrop Eight User Guide Thermo Scientific...
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