4
Nucleic Acid Applications
Measure dsDNA, ssDNA or RNA
Thermo Scientific
NanoDrop Eight User Guide 23
Reported Values
•
Nucleic acid concentration
. Reported in selected
unit (i.e., ng/µL, µg/µl or µg/mL). Calculations are based
on modified Beer’s Law equation using corrected nucleic
acid absorbance value.
•
A260/A280 purity ratio
. Ratio of corrected
absorbance at 260 nm to corrected absorbance at
280 nm. An A260/A280 purity ratio of ~1.8 is generally
accepted as “pure” for DNA (~2.0 for RNA). Acidic
solutions may under represent the reported value by
0.2-0.3; the opposite is true for basic solutions.
•
A260/A230 purity ratio
. Ratio of corrected
absorbance at 260 nm to corrected absorbance at
230 nm. An A260/A230 purity ratio between 1.8 and 2.2
is generally accepted as “pure” for DNA and RNA.
Note
: Although purity ratios are important indicators of
sample quality, the best quality indicator quality is
functionality in the downstream application of interest (e.g.,
real-time PCR).
•
Factor
. Used in conjunction with Beer’s Law to calculate
sample concentration
•
Contaminant
- If a contaminant was identified by the
Acclaro software, the contaminant will be displayed in this
column.
•
Species
- The species of nucleic acid contaminant
detected by the Acclaro sample intelligence technology
•
A260 absorbance
.
•
A280 absorbance
.
•
Baseline correction
•
Monitor wavelength
- Enter an additional wavelength
whose absorbance value you want included in the report.
•
Corrected
- Displays the corrected analyte
concentration determined using the Acclaro software, if
one is available.
Summary of Contents for NanoDrop Eight
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