ThermoFisher Scientific NanoDrop Eight, User Manual

Page: 28 / 152

4 Nucleic Acid Applications
Measure using a Custom Factor
28 NanoDrop Eight User Guide Thermo Scientific
Related Topics
• Basic Instrument Operations
• Nucleic Acid Reported Results
• Nucleic Acid Calculations
Settings for Nucleic Acid Measurements using a Custom Factor
To show the Custom Factor settings, from the Custom Factor measurement screen,
select the settings icon
to view the Custom Factor Setup .
Related Topics
• Instrument Settings
Setting Available Options Description
Custom Factor Enter an integer value
between 15 ng-cm/µL
and 150 ng-cm/µL Constant used to calculate nucleic acid concentration in
modified Beer’s Law equation . Based on extinction
coefficient and pathlength:
f = 1/(

260
* b))
where:
f = factor

= molar extinction coefficient at 260 nm in ng-cm/µL
b
= sample pathlength in cm (1 cm for nucleic acids
measured with the NanoDrop Eight instruments)
Baseline Correction On or off
Enter baseline
correction wavelength
in nm or use default
value (340 nm)
Optional user-defined baseline correction . Can
be used to correct for any offset caused by light scattering
particulates by subtracting measured absorbance at
specified baseline correction wavelength from absorbance
values at all wavelengths in sample spectrum. As a result,
absorbance of sample spectrum is zero at specified
baseline correction wavelength.
NOTE: Baseline correction is selected from the
measurement screen of the instrument software and is not
shown in the Custom Factor Setup.