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6
Custom Applications
Measure UV-Vis
58 NanoDrop Eight User Guide
Thermo Scientific
Measure UV-Vis
Measure UV-Vis
The UV-Vis application allows the instrument to function as a conventional
spectrophotometer. Sample absorbance is displayed on the screen from 190 nm to
850 nm. Up to 40 wavelengths can be designated for absorbance monitoring and
inclusion in the report. Automatic pathlength adjustment and a single-point baseline
correction can also be used.
To make UV-Vis measurements
Before you begin...
Before taking pedestal measurements with the NanoDrop Eight instrument, lift the
instrument arm and clean the upper and lower pedestals. At a minimum, wipe the
pedestals with a new laboratory wipe. For more information, see
To measure a sample using the UV-Vis application
1. From the Home screen, from the
Custom
tab, select
UV-Vis
.
(or you can specify them later if desired)
and whether automated pathlength adjustment, analysis wavelength, and
baseline correction will be used.
3. Pipette 1–2 µL blanking solution onto the lower pedestal and lower the arm.
Measures the absorbance of any
sample at up to 40 wavelengths
across the ultra-violet (UV) and visible
regions of the spectrum.
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will
flow into the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will
permanently damage the quartz fiber optic cables.
Summary of Contents for NanoDrop Eight
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