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6
Custom Applications
Measure Custom
Thermo Scientific
NanoDrop Eight User Guide 69
–
View and adjust the method settings as desired
–
Select
Save
Custom method settings
These settings are available for creating custom methods.
Setting
Available Options
Result name
Enter descriptive name for calculated concentration result (for example, “MTT
Assay”) and use adjacent drop down list to select appropriate unit. Result
name appears as column heading for reported concentration value.
Measurement range
Select spectral range in which method will acquire data.
Available options:
• Ultra-violet only (190 nm - 350 nm)
• Visible only (350 nm - 850 nm)
• Ultra-violet and visible (190 nm - 850 nm)
• Custom (specify starting and ending point in nanometers)
Notes
:
• If a
and/or
Analysis wavelength correction
are used,
make sure your selected spectral range includes your specified baseline
correction and/or analysis correction wavelength.
• For micro-volume absorbance measurements and measurements taken
with nonstandard (other than 10 mm) cuvettes, the spectra are normal-
ized to a 10 mm pathlength equivalent.
Analysis wavelength
correction
Use this option to specify absorbance correction at analysis wavelength only.
Available options:
•
None.
No correction at analysis wavelength.
•
Single point
. Enter wavelength for analysis correction. (Absorbance
value at specified analysis correction wavelength is subtracted from
absorbance value at analysis wavelength. Corrected value is used to
calculate sample concentration.)
•
Sloping baseline
. Enter two wavelengths that define sloping baseline
for analysis correction. (Absorbance value of sloping baseline at analysis
wavelength is subtracted from absorbance value at analysis wavelength.
Corrected value is used to calculate sample concentration.)
Summary of Contents for NanoDrop Eight
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Page 56: ...5 Protein Applications Measure Protein A280 56 NanoDrop Eight User Guide Thermo Scientific...
Page 76: ...6 Custom Applications Measure Custom 76 NanoDrop Eight User Guide Thermo Scientific...
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