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4
Nucleic Acid Applications
Measure dsDNA, ssDNA or RNA
18 NanoDrop Eight User Guide
Thermo Scientific
• Ensure the sample absorbance is within the instrument’s
• Blank with the same buffer solution used to resuspend the analyte of interest.
The blanking solution should be a similar pH and ionic strength as the analyte
solution.
• Run a
to assess the absorbance contribution of your buffer
solution. If the buffer exhibits strong absorbance at or near the analysis
wavelength (typically 260 nm), you may need to choose a different buffer or
application. See
Choosing and Measuring a Blank
for more information.
• For micro-volume measurements:
–
Ensure pedestal surfaces are properly
and
–
If possible, heat highly concentrated or large molecule samples, such as
genomic or lambda DNA, to 63 °C (145 °F) and gently (but thoroughly) vortex
before taking a measurement. Avoid introducing bubbles when mixing and
pipetting.
–
Follow
best practices for micro-volume measurements.
–
Use a 1-2 µL sample volume. See
for more
information.
Related Topics
• Measure a Micro-Volume Sample
• Best Practices for Micro-Volume Measurements
Note
Extraction reagents such as guanidine, phenol, and EDTA contribute
absorbance between 230 nm and 280 nm and will affect measurement
results if present in samples (even residual amounts).
Summary of Contents for NanoDrop Eight
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