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Contents
iv NanoDrop Eight User Guide
Thermo Scientific
Measure Oligo DNA or Oligo RNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31
Measure Oligo DNA or Oligo RNA . . . . . . . . . . . . . . . . . . . . . . . . . . .31
Oligo Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
Settings for Oligo DNA and Oligo RNA Measurements . . . . . . . . . . . .35
Detection Limits for Oligo DNA and Oligo RNA Measurements. . . . . .36
Calculations for Oligo DNA and Oligo RNA Measurements. . . . . . . . .37
Protein Applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
Measure Protein A280. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
Measure Protein Concentration at A280 . . . . . . . . . . . . . . . . . . . . . .41
Best practices for protein measurements . . . . . . . . . . . . . . . . . . . . . .43
Protein A280 Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45
Settings for Protein A280 Measurements . . . . . . . . . . . . . . . . . . . . . .46
Protein editor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49
Detection Limits for Protein A280 Measurements. . . . . . . . . . . . . . . .51
Calculations for Protein A280 Measurements . . . . . . . . . . . . . . . . . . .51
Custom Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57
Measure UV-Vis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58
Best practices for UV-Vis measurements . . . . . . . . . . . . . . . . . . . . . .59
UV-Vis Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
Settings for UV-Vis Measurements. . . . . . . . . . . . . . . . . . . . . . . . . . .62
Measure Custom. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63
Measure using a Custom Method . . . . . . . . . . . . . . . . . . . . . . . . . . .63
Delete Custom Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64
Custom Method Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . .65
Manage Custom Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .66
Learning Center . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77
Micro-Volume Sampling—How it Works . . . . . . . . . . . . . . . . . . . . . . . .78
Set Up the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80
Connect Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80
Connect to a Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80
Operating Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81
Measure a Micro-Volume Sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82
Best practices for micro-volume measurements. . . . . . . . . . . . . . . . .82
Recommended sample volumes . . . . . . . . . . . . . . . . . . . . . . . . . . . .83
Sample Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86
Prepare Samples and Blanks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86
Preparing Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
Run a Blanking Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90
Summary of Contents for NanoDrop Eight
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Page 56: ...5 Protein Applications Measure Protein A280 56 NanoDrop Eight User Guide Thermo Scientific...
Page 76: ...6 Custom Applications Measure Custom 76 NanoDrop Eight User Guide Thermo Scientific...
Page 142: ...8 Maintenance 142 NanoDrop Eight User Guide Thermo Scientific This page is intentionally blank...