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Learning Center
Measure a Micro-Volume Sample
82 NanoDrop Eight User Guide
Thermo Scientific
Measure a Micro-Volume Sample
Best practices for micro-volume measurements
Cleaning pedestals for daily operation
• Before first measurement, clean all pedestals with a new laboratory wipe.
•
to verify pedestals are clean.
• After each measurement, clean all pedestals with new wipe to prevent carryover.
• After each set of measurements, clean pedestals with DI H2O (see
)
•
periodically to maintain their hydrophobic property.
Pipetting Samples
• Position the instrument at an angle for optimal use of the pipette guide.
• Use calibrated precision pipettor (0–2 µL volume range) with well-fitting,
low-retention precision tips to apply sample material to instrument for
measurement. If using low accuracy (0-10 µL) pipettor, use 2 µL sample
volumes.
The NanoDrop Eight spectrophotometer uses surface
tension to hold a small volume of sample between two
pedestals. The patented sample retention system enables
the measurement of highly concentrated samples without
the need for dilutions. See
Supplies needed
• NanoDrop Eight spectrophotometer
• lint-free laboratory wipes
• calibrated precision pipettor
• sample material resuspended in appropriate buffer
solution (see
)
• pure buffer solution for blanking instrument (see
Summary of Contents for NanoDrop Eight
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