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7
Learning Center
Prepare Samples and Blanks
Thermo Scientific
NanoDrop Eight User Guide 91
1. From the Home screen, select an
application name.
2. Lift the instrument arm and clean the
upper and lower pedestals with new
laboratory wipe.
3. Measure a water blank:
–
Pipette exactly 1 µL deionized
water (DI H
2
O) onto the lower
pedestal and lower the arm.
–
Select
Blank
and wait for the
measurement to complete.
–
Lift the arm and clean all pedestals
with new laboratory wipe.
4. Measure the buffer solution:
–
Pipette 1-2 µL buffer solution onto
the pedestal and lower the arm.
–
Start the sample measurement:
–
if
arm
–
if Auto-Measure is off, lower
arm and select
Measure
–
Wait for measurement to complete.
The resulting spectrum should vary no
more than 0.04 A from the baseline at
the analysis wavelength.
If your spectrum does not meet these
criteria, repeat steps 2–4.
If spectrum is still outside
specifications, see
5. When you are finished with the
blanking cycle, select
End Experiment
.
6. Lift the arm and clean all pedestals
with a new wipe.
Example spectrum of buffer suitable
for Protein A280 protein quantification
Example spectrum of buffer unsuitable
for Protein A280 protein quantification
Summary of Contents for NanoDrop Eight
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Page 56: ...5 Protein Applications Measure Protein A280 56 NanoDrop Eight User Guide Thermo Scientific...
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