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6
Custom Applications
Measure UV-Vis
Thermo Scientific
NanoDrop Eight User Guide 59
4. Select
Blank
and wait for the measurement to complete.
Tip
: If
is On, the blank measurement starts automatically after you
lower the arm.
5. Lift the arm and clean all pedestals with a new laboratory wipe, .
6. Pipette 1-2 µL sample solution onto the pedestal and lower the arm.
7. Start the sample measurement: If
if Auto-Measure is off, lower arm and select
Measure
.
When the sample measurement is completed, the spectrum and reported values
are displayed (see the next section).
8. When you are finished measuring samples, select
End Experiment
.
9. Lift the arm and clean all pedestals with a new wipe.
Best practices for UV-Vis measurements
• Ensure the sample absorbance is within the instrument’s
• Blank with the same buffer solution used to re-suspend the analyte of interest.
The blanking solution should be a similar pH and ionic strength as the analyte
solution.
• Run a
to assess the absorbance contribution of your buffer
solution. If the buffer exhibits strong absorbance at or near an analysis
wavelength, you may need to choose a different buffer or application. See
Choosing and Measuring a Blank
for more information.
• For micro-volume measurements:
–
Ensure pedestal surfaces are properly
and
–
Ensure samples are homogeneous before taking a measurement. Avoid
introducing bubbles when mixing and pipetting.
–
Follow
best practices for micro-volume measurements.
–
Use a 1-2 µL sample volume. See
for more
information.
Summary of Contents for NanoDrop Eight
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