ThermoFisher Scientific NanoDrop Eight, User Manual

Page: 83 / 152

7
Learning Center
Measure a Micro-Volume Sample
Thermo Scientific NanoDrop Eight User Guide 83
• Use recommended sample volumes to ensure proper liquid column formation.
• Use new tip for each blank and sample aliquot.
• Use new aliquot of sample for each measurement.
• When the measurement is complete, open the sampling arm and wipe the
samples from both the upper and lower pedestals using a soft laboratory wipe.
If solvents are used, make sure they are compatible with the pedestals. (see
“Compatible Solvents” in
Hazardous Materials ).
Recommended sample volumes
To measure a micro-volume sample
Application Sample Volume
Nucleic acid (aqueous solution) 1 µL
a
a
Use 2 µL for samples that contain materials that may reduce surface tension such as a surfactant.
Purified protein 2 µL
Other protein applications such as Bradford or BCA 2 µL
Microbial cell suspensions 2 µL
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will
flow into the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will
permanently damage the quartz fiber optic cables.
1. From the Home screen, select an application from
one of the application categories, such as UV-Vis , or
Custom Methods.