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8
Maintenance
Maintaining the Pedestals
Thermo Scientific
NanoDrop Eight User Guide 131
Reconditioning the Pedestals
The pedestal surfaces may lose their “conditioned” properties over time, especially
after measurements with isopropyl alcohol or solutions that contain surfactants or
detergents such as the Bradford reagent. An unconditioned pedestal causes
droplets on the lower pedestal to “flatten out,” preventing proper formation of the
liquid column when the arm is lowered. The resulting spectrum may look “rough” or
“jagged.”
If samples flatten out on the pedestal (rather than “beading up” or forming a rounded
droplet) or the liquid column breaks during a measurement, recondition the
pedestals.
Supplies needed
• lint-free laboratory wipes
•
PR-1 pedestal reconditioning kit
(available from us or a local distributor)
• calibrated precision pipettor (0-2 µL)
• canned air
Unconditioned pedestal
(droplet flattens out)
Properly conditioned pedestal
(droplet beads up)
Summary of Contents for NanoDrop Eight
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Page 56: ...5 Protein Applications Measure Protein A280 56 NanoDrop Eight User Guide Thermo Scientific...
Page 76: ...6 Custom Applications Measure Custom 76 NanoDrop Eight User Guide Thermo Scientific...
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