7
Learning Center
Prepare Samples and Blanks
88 NanoDrop Eight User Guide
Thermo Scientific
• Run a
to assess the suitability
of your blanking solution before using it to
perform sample measurements. For a quick
demonstration, watch the multimedia training
Evaluating a Blanking Solution for Suitability
The resulting spectrum should vary no more
than 0.04 A (10 mm equivalent) across the
spectrum, especially at the analysis
wavelength as in the example at the right.
If the resulting spectrum is greater than
0.04 A around the analysis wavelength, that
buffer solution may interfere with the sample
analyses, especially for low concentration
samples. See below for details.
Good blanking buffer (measured abs < 0.04)
Problems associated with blanking
• Residual sample was left on pedestal before
blank measurement was performed.
(Resulting sample spectra may exhibit
negative absorbance values, indicating blank
had more absorbance than sample in that
region of spectrum.)
• Blank measurement exhibits higher
absorbance than unknown sample at
analysis wavelength. (If buffer used as blank
differs in composition from that used to
resuspend sample, measurement results will
be incorrect.)
• Sample was inadvertently used to blank
instrument. (Resulting sample spectra may
exhibit negative absorbance values or, in
some cases, resemble a mirror image of a
typical pure nucleic acid or protein
spectrum.)
Solution containing salt used to blank instrument results
in “mirror image” spectrum
Summary of Contents for NanoDrop Eight
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