ThermoFisher Scientific NanoDrop Eight, User Manual

Page: 72 / 152

6 Custom Applications
Measure Custom
72 NanoDrop Eight User Guide Thermo Scientific
Analysis wavelength
(Standard curve methods
only) Monitor absorbance at specified wavelength (enter the wavelength in nano-
meters).
Note : The specified wavelength must fall within the selected measurement
range .
The measurement results or the concentration will be calculated automatically
using the absorbance value at the specified wavelength and applying the
selected method type (factor or standard curve).
Baseline correction Select this option to correct offset caused by light scattering particulates by
subtracting the absorbance at a specified baseline point. Then specify
wavelength for baseline correction.
Note : Software subtracts absorbance value at specified baseline correction
wavelength from absorbance values at all wavelengths in sample spectrum.
As a result, absorbance of sample spectrum is zero at specified baseline
correction wavelength.
Automated pathlength Affects micro-volume measurements only.
• When Automated Pathlength is selected, software selects the optimal
pathlength (between 1.0 mm and 0.1 mm) based on sample absorbance
at the analysis wavelength. For example, when sample absorbance at the
analysis wavelength is less than or equal to 12.5 (10 mm pathlength
equivalent), the optimal longer pathlength is used. When sample
absorbance is greater than 12.5, the optimal shorter pathlength is used.
Recommended for samples that are highly absorbing at the analysis
wavelength. (This option may cause reduced sensitivity when the sample
spectra have a large absorbance peak that is not at the analysis
wavelength.)
Note : When the analysis wavelength is between 190 nm and 219 nm,
the optimal longer pathlength is used when sample absorbance is less
than or equal to 10 (10 mm pathlength equivalent), and the optimal
shorter pathlength is used when sample absorbance is greater than 10.
• When Automated Pathlength is deselected, the software uses a 1 mm
pathlength regardless of the sample absorbance. This can cause detector
saturation (resulting in jagged peaks) for highly absorbing samples (e.g.,
~15 A at 10 mm pathlength equivalent).
Setting Available Options