5
Protein Applications
Measure Protein A280
46 NanoDrop Eight User Guide
Thermo Scientific
• Baseline correction absorbance
• created on (date sample measurement was taken)
• monitor wavelength
Related Topics
Settings for Protein A280 Measurements
To show the Protein A280 settings, from the Protein A280 measurement screen,
select the settings icon
to view
Protein A280 Setup
.
Protein A280 settings
The Protein A280 application provides a variety of sample type options for purified
protein analysis.
Each sample type applies a unique extinction coefficient to the protein calculations. If
the extinction coefficient of the sample is known, choose the
+ MW (molar) or
1%
(mass) option and enter the value. Otherwise, calculate the extinction coefficient or
choose the option that best matches the sample solution. If you only need a rough
estimate of protein concentration and the sample extinction coefficient is unknown,
select the 1 Abs=1 mg/mL sample type option.
Summary of Contents for NanoDrop Eight
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