ThermoFisher Scientific NanoDrop Eight, User Manual

Page: 71 / 152

6
Custom Applications
Measure Custom
Thermo Scientific NanoDrop Eight User Guide 71
Standards (Standard
curve methods only) Define the standards:
• Enter name and analyte concentration of each standard and a reference,
if desired:
– Depending on the Curve Type setting, a standard curve can be
generated using two or more standards. (The software
allows a
reference and up to 7 standards .)
– All reference and standards solutions should be in the same
buffer used to resuspend the samples plus the same volume of
reagent added to the samples.
– First standard can be a reference measurement. The reference
solution should contain none of the analyte of interest. (The reference
measurement is not the same as a blank measurement.)
– Concentration values for standards can be entered in any
order but the standards must be measured in the order in which they
were entered; however, best practice dictates that standards be
measured from the lowest concentration of the standard analyte
stock to the highest.
– Concentration range of the standards must cover the
dynamic range of the assay and the expected range of the unknown
samples. Sample analyte concentrations are not extrapolated beyond
the concentration of the highest standard.
• Select curve fit type.
Specify type of equation used to create standard curve from standard
concentration values. Available options:
– Linear: Draws the linear least squares line through all measured
standards (requires reference measurement and at least one
standard)
– Interpolation: Draws a series of straight lines to connect all measured
standards (requires reference measurement ans at least one
standard)
– 2
nd
order polynomial: Draws the 2
nd
order least squares polynomial
using all measured standards (requires reference measurement and at
least standards)
– 3
rd
order polynomial: Draws the 3
rd
order least squares polynomial
using all measured standards (requires reference measurement and at
least three standards)
Setting Available Options