Thermo Scientific VF-F20-A, Operating Manual

Page: 105 / 240

6
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Operation
Integral Fraction Collector FT (VF-F20-A) Operating Manual Page 105
6.5 Preparing the Device for Operation
This section gives information on any additional steps that are required
to prepare the device for operation.
Before Operating the Device for the First Time
Prepare the device for the first-time operation, observing the following:
NOTICE Flush the system flow path thoroughly before operating the
device for the first time:
•
When you install devices or components to the system, always flush
them to waste before connecting them in the system flow path. To
flush the Vanquish modules, follow the instructions in the Vanquish
System Operating Manual.
•
Some components of the device are filled with isopropanol when the
device is shipped from the manufacturing site. When operating the
device for the first time, use solvents that are miscible with
isopropanol. If they are not, use an appropriate intermediate
solvent.
•
Inspect components and fluidic connections prior to fluidic
operation.
1. Verify that all necessary solvents are available in the solvent rack
and properly connected to the device.
2. Verify that waste line has been properly directed to waste
container.
Before Starting Fraction Collection
•
Check the liquid level in the solvent reservoirs. Verify that the
amount of solvent is sufficient for the collection.
•
Load the carousel with well plates or racks with empty.
NOTICE Before starting a sample or sequence, verify that the rack
types set in Chromeleon match the rack types in the fraction
collector compartment.