Thermo Scientific VF-F20-A, Operating Manual

Page: 122 / 240

6
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Operation
Page 122 Integral Fraction Collector FT (VF-F20-A) Operating Manual
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Do not pull the tubing (transparent waste line, O D 1/8’’) connecting
with the faction valve port 3, on which an air bubble sensor is
installed.
6.7.3 How to Perform DVD Function
The product provides an automated Delay Volume Determination
feature by running the Delay Volume Determination test case on
Chromeleon 7 Console : Click Tools > Instrument Qualification >
Instrument Qualification Wizard > Select Operational Qualification >
Choose an Instrument > Select General > Select Vanquish Fraction
Collector Delay Volume Determination.
Then follow below steps to complete DVD function.
NOTICE
Prior to performing DVD flush the whole system with pure water to
remove air from the fluidic path.
1. Use purified water as mobile phase for running DVD function.
2. The procedure requires user intervention after 1 min to install the
DVD capillary (included in ship kit, P/N 6040.2330, Viper Cap. ID x L:
0.13 x 850 mm, SST) and put an empty vial at position R :A1 issued
by respective messages in Chromeleon.
3. After the DVD capillary was installed and the empty vial was put at
R:A1 the remaining sequence runs automatically.
4. The overall procedure takes about 30 minutes.
NOTICE
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If the DVD is applied during daily application instead of the first
time system installation, the capillaries between autosampler and
detector as well as the column need to be uninstalled.
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The DVD capillary connects autosampler and detector directly.
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Make sure the column is bypassed.
5. After the DVD is performed, users can check the measured Delay
Volume in Chromeleon > FC ePanel tab > More Options dialog .