Thermo Scientific VF-F20-A, Operating Manual

Page: 140 / 240

6
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Operation
Page 140 Integral Fraction Collector FT (VF-F20-A) Operating Manual
Situation after Shutdown If no additive is used If an addititive is used
Device shall be transported
or shipped after shutdown Flush the system with
isopropanol. Flush the system first with
several volumes (for
example, 1.0 mL/min for 10
minutes with the standard
system) of methanol and
water (50:50) to prevent
salt buildup in the fluidics. If
the solvents in the device
are not miscible with water,
use an appropriate
intermediate solvent.
Afterward, flush the system
with isopropanol.
1. Set the fraction collection valve to Waste position.
2. Turn off the pump flow to the device. Wait until the system pressure
is down to zero before you continue the shutdown of the device.
3. If the device shall be transported or shipped after shutdown

Flush the needle wash system with an organic needle wash
solvent, such as isopropanol, before you interrupt operation to
avoid organic growth in the needle wash system.

Purge flush pump to flush buffer loop with an organic solvent,
such as isopropanol, before you interrupt operation to avoid
organic growth in the flush buffer loop.

Run an internal needle rinse cycle with an organic solvent, such
as isopropanol, before you interrupt operation to avoid organic
growth inside the needle capillary and needle of the fraction
collector.
4. Empty condensation drain system:
a) Leave the drain pump turned on until no condensation water is
present in the waste tubing of the drain pump. Then turn off the
drain pump.
b) Remove the sample racks and sample containers from the
fraction collector compartment.
c) If condensation or spilled samples are present in the fraction
collector compartment, clean and decontaminate the fraction