Thermo Scientific VF-F20-A, Operating Manual

Page: 217 / 240

9
•
Specifications
Integral Fraction Collector FT (VF-F20-A) Operating Manual Page 217
9.1 Performance Specifications
The fraction collector performance is specified as follows:
Type Specification
Operating Specifications
Sample capacity
•
Any four of the following sample racks or well plates with SBS footprint
format
•
54 x 12 mm O.D. vials ( ≤ 1.5 mL)
•
96 x 6, 7 and 8 mm O.D. vials ( ≤ 1.2 mL)
•
16 x 15 mm O.D. vials ( ≤ 4 mL)
•
9 x 22.5 mm O.D. vials ( ≤ 10 mL)
•
96- and 384-position well plates (deep and shallow)
Collection Modes
•
Triggered by peak signals or time frames
•
(Saw)vertical, (saw)horizontal
•
Interrupt and continuous
•
Above or in sample container
•
Manual collection, pooling, wrapping and concatenating
Needle Wash
•
Rinse of inner needle surface and needle capillary with mobile phase into
needle wash port (before or after each injection)
•
Dip wash and continuous wash of outer needle surface
•
Flush function between fractions to flush inner needle surface and needle
capillary with flush liquid into sample container
Operating Flow Range 0.05 mL/min – 10 mL/min
Delay Volume ~ 19 µL with
•
Fraction collector inlet to fraction collection valve port: ~ 2.7 µL (default,
time-based fractionation, one stack configuration)
•
Fraction collection valve: < 0.5 µL
•
Fraction collection valve to needle: ~ 10.7 µL (default)
•
Needle: ~ 5 µL
Min. Collection Volume
One droplet collection volume:
≥
6µL water
Carry over Carry over < 0.15% with flush enabled
Automation Features
•
Barcode reading:

Empty segment detection

Rack/well plate verification

Inventory management
•
Fraction simulation to model and optimize peak detection options in
Chromeleon CDS using chromatogram data.
Temperature Range 4 – 40 °C, cooling
≥
23 K below ambient at < 80% relative humidity