Thermo Scientific VF-F20-A, Operating Manual

Page: 213 / 240

8
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Troubleshooting
Integral Fraction Collector FT (VF-F20-A) Operating Manual Page 213
8.3.2 Resolving Clogging in the Device
When
Components in the device flow path are clogged
Preparations
Turn off the pump flow to the device. Wait until the system pressure is
down to zero. Be sure that no backpressure from other modules in the
system is present.
Follow these steps
Turn off pump flow and wait for the system pressure drop down. Bypass
the detector with DVD capillary (P/N: 6040.2330, ID x L: 0.13 × 850 mm).
The fluidics connection is shown as below:
1. Press the Service button on the keypad. The device moves the
needle unit into service position.
2. Disconnect the needle capillary fitting from the needle unit.
3. Place the open connector of the needle capillary into the central
hole of needle wash port.
4. Check that the fraction collection valve is set to collection position.
5. In Chromeleon, set the pump flow rate to the maximum pump flow
rate.
6. Turn on the pump flow and flush the needle capillary to wash port at
the maximum pump flow rate for approximately 1 minute.
Afterward,
turn off the pump flow .
7. Connect the needle capillary to the needle unit. Before tightening
the needle capillary, position it correctly ( refer to Replacing the
Needle Capillary (

Page 172) .
8. Press Wash button to move the needle unit into the needle wash
port position.
9. Turn on the pump flow and rinse the internal surface of needle
capillary and needle into the needle wash port at the maximum
pump flow rate for approximately 1 minute. (Make sure the fraction
collection valve remains at the collection position);