Qiagen PyroMark Q24 MDx, User Manual

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Operating Procedures
5-26 PyroMark Q24 MDx User Manual 01/2016
Quality colors
Blue: Passed
Yellow: Check
Red: Failed
White: Not analyzed*
* Either analysis is not supported by the software (e.g., analysis of SNP when in
the CpG mode) or the variable position has been deselected by the user (AQ
and CpG assays only).
AQ analysis results
The allele frequencies are displayed in Pyrogram, for
example
and (InDel). The quality assessment is
displayed by the background color of the result.
2% 1% 4% 1% 3%
E E S S G G T T C C G G A A
5 C C T T A A T T G G
10
T T C C G G A A T T
15
T T G G A A T T C C
20
A A G G T T C C G G
25
T T A A T T G G T T
30
C C G G T T A A
0
100
200
300
400
A5: YGGATAGYGATTTTTAAYGYGTAAGYGTATA
Example Pyrogram for a CpG assay. Variable positions in AQ and
CpG assays are highlighted with a blue-gray background color, and
bisulfite treatment controls in CpG assays with a light yellow
background color.
CpG analysis results
The methylation percentages are displayed in Pyrogram, for
example . The quality assessment is displayed by the
background color of the result.