Qiagen PyroMark Q24 MDx, User Manual

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Glossary
8-4 PyroMark Q24 MDx User Manual 01/2016
Term Description
Quality control
window
Gives an overview of the quality at the end of a defined
number of bases in the base-called sequence. A setting in
the SQA analysis mode.
Reference peak Nonvariable peaks (i.e., peaks that are not a part of a
variable position) are referred to as "reference peaks".
Reference peaks are used in the analysis both as
references when calculating the single peak height level,
and as internal controls when assessing the quality.
RLU
Relative Light Unit (entity used in Pyrosequencing to define
peak heights in Pyrogram).
Sepharose
beads
Streptavidin-coated beads should be used for preparation
of biotinylated PCR products.
SQA
Analysis mode used for base-calling of unknown
sequences.
Sequence to
analyze
A short part of a DNA sequence (in your sample), starting
directly after the sequencing primer, which contains one
or several variable positions to be analyzed using
Pyrosequencing instrument platforms.
Sequencing
primer The sequencing primer is annealed to the template during
the sample preparation. The 3'-end of the sequencing
primer serves as the starting point for the extension by the
DNA polymerase.
Shift
Plus shift: A small proportion of the template sequences
that incorporates more than one type of nucleotide at a
time (if, for example, there are residues left from the
dispensation before) and will be sequenced ahead of the
rest of template sequences.
Minus shift: A small proportion of the template sequences
that fails to incorporate a nucleotide will be sequenced
subsequent to the rest of template.