Qiagen PyroMark Q24 MDx, User Manual

Page: 79 / 104

Glossary
PyroMark Q24 MDx User Manual 01/2016 8-3
Term Description
IUPAC
International Union of Pure and Applied Chemistry. An
organization providing recommendations on organic and
biochemical nomenclature, symbols, terminology, etc.
IUPAC codes
A = Adenine W = T or A
C = Cytosine S = C or G
G = Guanine B = C, T, or G (not A)
T = Thymine D = A, T, or G (not C)
R = Purine (A or G) H = A, T, or C (not G)
Y = Pyrimidine (C or T) V = A, C, or G (not T)
M = C or A N = Any base (A, C, G, or T)
K = T or G
Out of phase When one of the alleles is sequenced ahead of the other.
Polymorphism
Genetic variations, broadly encompassing any of the
many types of variations in DNA sequences that are
found within a given population.
Presequencing
signal
As the substrate is dispensed into a well (indicated by an
“S” in the Pyrogram), contaminants (such as ATP or PPi)
will be converted to light. Too high a substrate peak
indicates that high levels of a contaminant might be
present in, for example, buffers.
Pyrogram
The graph resulting from a sequencing reaction
performed using Pyrosequencing technology.
Incorporated nucleotides are shown as peaks in the
Pyrogram.