Qiagen PyroMark Q24 MDx, User Manual

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Operating Procedures
5-16 PyroMark Q24 MDx User Manual 01/2016
5.3.5 Annealing of sequencing primer to samples
WARNING Hot surface
The plate holder and the heating block (for annealing) can
reach temperatures of up to 80°C (176°F). Avoid touching
them when they are hot.
1. Heat the PyroMark Q24 Plate containing the samples at
80°C for 2 min using the PyroMark Q24 Plate Holder
(two are supplied with the vacuum workstation) and a
heating block.
2. Remove the plate from the plate holder and allow the
samples cool to room temperature (15–25°C) for at least
5 min. The plate can now be processed in the PyroMark
Q24 MDx Instrument.
5.4 Preparation of PyroMark Gold Q24
Reagents
WARNING Sharp needles
Do not touch the sharp needles at the bottom of the
reagent cartridge. Handle the needles with care. Small
particles and fibers may obstruct the needles.
1. Open the PyroMark Gold Q24 Reagents box and
remove the vials containing freeze-dried enzyme and
substrate mixtures, and the tubes containing nucleotides.
2. Reconstitute the volumes of reagents required and fill
PyroMark Q24 Cartridge according to the handbook
supplied with the reagents.
Note : The required volumes of reagents are listed in the
“Pre Run Information” report (see Section 5.2.3).
Note : Ensure any reused reagent cartridges are cleaned
thoroughly, according to the instructions in Section 5.5.5.
It is recommended that the reagent cartridge is used a
maximum of 30 times. If the reagent cartridge has not
been used in 4 weeks or longer (e.g., it has been stored),
clean the cartridge and check that it can be used for