Qiagen PyroMark Q24 MDx, User Manual

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Operating Procedures
5-12 PyroMark Q24 MDx User Manual 01/2016
The mains plug should be easily accessible in case the
vacuum pump needs to be disconnected quickly from the
mains power.
Note : Perform the function test to ensure that the filter
probes are working properly (see Section 5.3.1). All filter
probes should be replaced after preparation of
approximately 100 plates.
Note : Empty the waste container if necessary (see
Section 5.6.2).
2. Fill five separate troughs supplied with the PyroMark
Q24 MDx Vacuum Workstation as follows:

Approximately 50 ml ethanol (70%) (1)

Approximately 40 ml Denaturation Solution (2)

Approximately 50 ml 1x Wash Buffer (3)

Approximately 50 ml high-purity water (4)

Approximately 70 ml high-purity water (5)
A suggested setup is shown in the following image. Refill
the troughs to these levels whenever necessary.