Qiagen PyroMark Q24 MDx, User Manual

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Glossary
PyroMark Q24 MDx User Manual 01/2016 8-1
8 Glossary
Term Description
AQ Analysis mode used for quantification of different alleles.
Biotin
A molecule that can bind very strongly to streptavidin.
PCR primers can be biotinylated to enable the resulting
PCR product to bind to streptavidin-coated beads.
Bisulfite
HSO
3
- is referred to as bisulfite (or hydrogen sulfite). In
the bisulfite reaction, DNA is treated with sodium bisulfite
to convert cytosine residues to uracil, under conditions
whereby methylated cytosines remain non-reactive.
Bisulfite
treatment
control
Pyrosequencing assays can contain an internal control to
assess successful bisulfite treatment. C bases that are not
followed by G in the sequence are normally not
methylated, and should therefore be fully converted to T
after bisulfite treatment and PCR. As a result of successful
bisulfite treatment, all templates should show only Ts and
no Cs in these positions. For reverse assays, all templates
should show only As and no Gs in these positions.
CpG Analysis mode used for analysis of CpG methylation.
Cyclic
dispensation
order
A repetitive dispensation order for nucleotide
dispensation. Normally used in Pyrosequencing
technology for sequencing unknown DNA-sequences. For
example, "CTGA" or "TCGA" can be used and repeated
for the desired number of times.
Directed
dispensation
order
Non-cyclic order of dispensation that follows the known
sequence. It can be used in Pyrosequencing technology
when you know the sequence to be analyzed. For
example, the sequence "TCCAGAA" can be analyzed with
the dispensation order "TCAGA".
Dispensation
order Defines the nucleotides and the order in which they
should be dispensed in Pyrosequencing runs.