Qiagen PyroMark Q24 MDx, User Manual

Page: 43 / 104

Operating Procedures
PyroMark Q24 MDx User Manual 01/2016 5-15
17. While holding the tool over the PyroMark Q24 Plate,
close the vacuum switch on the tool (Off).
18. Release the beads in the plate containing sequencing
primer, by shaking the tool gently from side to side.
19. With the vacuum switch closed (Off), transfer the tool to
the trough containing high-purity water and agitate the
tool for 10 s.
20. Wash the filter probes by lowering the probes into the
second trough of high-purity water and applying
vacuum. Flush the filter probes with 70 ml high-purity
water.
21. Raise the tool to beyond 90° vertical for 5 s, to drain
liquid from the filter probes.
22. Close the vacuum switch on the tool (Off) and place the
tool in the Parking (P) position.
23. If more than one plate is prepared at once, refill the
troughs (step 2) and repeat the procedure from step 8.
24. Turn off the vacuum pump.
25. At the end of a working day, liquid waste and any
remaining solutions should be discarded and the
PyroMark Q24 MDx Vacuum Workstation should be
checked for dust and spillage, (see Section 5.6.2).