Qiagen PyroMark Q24 MDx, User Manual

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Operating Procedures
PyroMark Q24 MDx User Manual 01/2016 5-9

PyroMark Q24 Cartridge

Strip caps

Streptavidin Sepharose High Performance (34 µm,
5 ml, GE Healthcare; see www.gelifesciences.com)

QIAGEN IVD-labeled assays for Pyrosequencing

High-purity water (Milli-Q 18.2 MΩ x cm or equivalent)

Ethanol (70%)

PyroMark Binding Buffer

PyroMark Denaturation Solution

PyroMark Wash Buffer concentrate

PyroMark Annealing Buffer
5.3.1 PyroMark Q24 MDx Vacuum Workstation
function test
Before using the PyroMark Q24 MDx Vacuum Workstation,
check that the filter probes are working properly by
performing the function test as follows:
1.
Add 100 µl high-purity water to each well of a 24-well
PCR plate.
2. Fill a trough with 70 ml high-purity water.
3. Start the vacuum pump.
4. Apply vacuum to the vacuum prep tool by opening the
vacuum switch.
5. Lower the filter probes into the trough. Keep them in
position for approximately 20 s. Ensure that the water is
transferred to the waste container, i.e., that vacuum has
been applied. If not, check the connections.
6. Lower the filter probes into the PCR plate and check that
the water is aspirated evenly across all wells and that all
wells are empty after a maximum of 10 s.
7. If the wells are not empty after 10 s, repeat the
procedure from step 1. If the function test fails twice,
replace the filter probes (see Section 6.3.2).