Qiagen PyroMark Q24 MDx, User Manual

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Contents
PyroMark Q24 MDx User Manual 01/2016 Contents-3
5.3.4 Separation of DNA strands and release of
samples into the PyroMark Q24 Plate 5-11
5.3.5 Annealing of sequencing primer to samples 5-16
5.4 Preparation of PyroMark Gold Q24 Reagents 5-16
5.5 Processing a run on the PyroMark Q24 MDx
Instrument 5-17
5.5.1 Instrument software 5-18
5.5.2 Starting the instrument 5-18
5.5.3 Starting the run 5-19
5.5.4 Monitoring the run 5-20
5.5.5 After the run 5-21
5.5.6 Analyzing the run 5-24
5.5.7 Viewing the analysis results 5-25
5.5.8 Analysis reports 5-27
5.6 Finishing work and shutting down 5-28
5.6.1 Shutting down the instrument 5-28
5.6.2 Emptying the waste container and troughs 5-29
5.6.3 Checking the instrument 5-30
5.7 Backup of PyroMark Q24 files 5-30
6 Maintenance 6-1
6.1 Checking the performance of the PyroMark Q24 MDx 6-1
6.2 Maintenance of the PyroMark Q24 MDx Instrument 6-2
6.2.1 Cleaning the instrument 6-2
6.2.2 Cleaning the heating block and light guides 6-3
6.3 Maintenance of the PyroMark Q24 MDx Vacuum
Workstation 6-5
6.3.1 Cleaning the PyroMark Q24 MDx Vacuum
Workstation 6-5
6.3.2 Testing and replacing the filter probes 6-6
6.3.3 Replacing the rubber seal 6-7
6.3.4 Replacing the tubing 6-8
6.3.5 Replacing the waste filter 6-9