Leica 14 9AUTO00C1 Instructions For Use Manual Download Page 64

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Version 1.1, Revision B

Operation

5.8

Sectioning

Warning

Personnel with insufficient qualification working with the instrument.

Serious injury of persons and or specimen damage can occur when specimen approaches knife/

blade due to inappropriate operator actions, for example, because object head may drop onto knife

holder when handwheel is unlocked.

• Always make sure that only laboratory personnel with dedicated and sufficient training and

qualification operates the instrument.

• Always make sure that all laboratory personnel designated to operate this instrument has read

these Instructions for Use carefully and is familiar with all technical features of the instrument

before start working with it.

Warning

Personal protective equipment is not used.

Injury of persons.

• When working with microtomes, personal safety precautions must always be taken. It is

mandatory to wear work safety shoes, safety gloves, a mask and safety goggles.

Warning

Working with the instrument and brittle specimen without appropriate protective clothes.

Serious injury of persons by splinters while cutting brittle specimen.

• Always wear appropriate protective clothes (including safety goggles) and work especially

carefully when cutting brittle specimen.

Warning

Insufficient frame conditions for sectioning.

Specimen damage or poor sectioning results,for example, sections alternating in thickness,

compressed, folded or stripped sections.

• Do not continue sectioning if you recognize insufficient sections results.

• Make sure that all prerequisites for appropriate sectioning are met. Refer to the troubleshooting

section of these Instructions for Use for further details.

• If you do not have sufficient knowledge in regards of troubleshooting insufficient sectioning

results consult persons who do have that knowledge, for example, application experts of Leica

Biosystems.

Warning

Turning the handwheel counterclockwise.

People injury/Damage to specimen

• Do not turn the handwheel counterclockwise, which may lead to handwheel locking mechanism

malfunction.

Summary of Contents for 14 9AUTO00C1

Page 1: ...UTOCUT Rotary Microtome Instructions for Use English Order No 14 0519 80101 Revision B Always keep this manual with the instrument Read carefully before working with the instrument Version 1 1 Revisio...

Page 2: ......

Page 3: ...ical details of the present Instructions for Use are not to be considered warranted characteristics of our products These are determined only by the contract provisions agreed between ourselves and ou...

Page 4: ...t Components and Specifications 25 3 1 Overview instrument components 25 3 2 Instrument specifications 26 3 3 Technical Data 28 4 Setting up the instrument 30 4 1 Installation site requirements 30 4 2...

Page 5: ...tine 66 6 Optional Accessories 68 6 1 Assembly for fixture for specimen clamps 68 6 1 1 Rigid fixture for specimen clamps 68 6 1 2 Directional fixture for specimen clamps 69 6 1 3 Fine directional fix...

Page 6: ...4 1 Replacing the pressure plate 107 7 4 2 Mounting the clamping top onto the segment arc 108 7 5 The setting of the clamping system of the clamp mount on the base plate 108 8 Cleaning and Maintenanc...

Page 7: ...the machine or something in its vicinity Tip Gives you tips to simplify your workflow Item numbers for numbering illustrations Fig 7 1 Numbers in red refer to item numbers in illustrations Manufacture...

Page 8: ...nternational NSF and others Symbol for alternating current PE terminal Power on Power off The package contents are fragile and must be handled with care The package must be kept in a dry environment M...

Page 9: ...ndicating the instrument serial number is attached at the back side of the instrument 1 3 Intended use The HistoCore AUTOCUT is a fully automatic motorized rotary microtome with a separate control pan...

Page 10: ...rious injury of persons and or property damage including specimen damage Never remove or modify any protective devices of the instrument or any accessories Only service personnel qualified by Leica Bi...

Page 11: ...touch it only at the lifting points described in the Instructions for Use and Unpacking Instructions base plate at the front and under the instrument on the back Never lift the instrument by the handw...

Page 12: ...o avoid accidental falling off Warning Section waste tray falling down after it has been detached Injury of persons Work especially carefully when detaching the section waste tray and put it in a safe...

Page 13: ...ion working with the instrument Serious injury of persons and or specimen damage can occur when specimen approaches knife blade due to inappropriate operator actions for example because object head ma...

Page 14: ...lade holder was uninstalled from the microtome Serious injury of persons due to contact with extremely sharp knife blade Before uninstalling the knife blade holder from the microtome always make sure...

Page 15: ...cutting edge facing upwards and never try to catch a falling knife Warning Uploading or unloading the specimen on the microtome without appropriate clothes and in an inappropriate manner Operator can...

Page 16: ...ning if you recognize insufficient sections results Make sure that all prerequisites for appropriate sectioning are met Refer to the troubleshooting section of these Instructions for Use for further d...

Page 17: ...hat you have centered the handle of the handwheel Warning When doing fast manual trimming and fingers are put between the specimen and the knife blade after the handwheel is released Operator can get...

Page 18: ...otating the handwheel and the coarse feed wheel at the same time Specimen damage Do not turn the coarse feed wheel whilst rotating the handwheel Caution The feather key is lost when mounting the handw...

Page 19: ...fast homing or moving to the memory position Caution The memory position is set too close to the knife blade Specimen impaired Make sure that the specimen does not touch the cutting edge of the knife...

Page 20: ...irection during cleaning Serious injury of persons Always wipe the knife from the back of the knife to the cutting edge Warning Removing the parts from the drying chamber 65 C during cleaning of the k...

Page 21: ...ctive devices Warning Protective devices or safety accessories provided by manufacturer have been removed or modified Serious injury of persons and or property damage including specimen damage Never r...

Page 22: ...he motorized sectioning use the RUN STOP button or ENABLE button or the BRAKE button on the separate control panel P 49 Start and stop motorized sectioning Using the handwheel brake lever Fig 2 3 on t...

Page 23: ...sectioning mode Activate the handwheel locking mechanism To center the handwheel lightly pull the handle Fig 4 1 outwards and swivel it into the center of the handwheel Fig 4 2 Release the handle and...

Page 24: ...der E consists of a red foldaway handle Fig 8 1 To cover the cutting edge fold the safety guard handle upwards as illustrated in Fig 8 Tip The clamping levers on the two in one blade holder E are not...

Page 25: ...tch 9 Socket for backlighting 2 Coarse feed wheel 10 Handwheel brake lever 3 Separate control panel 11 Object head with orientation and UCC 4 Microtome base 12 Handwheel 5 Blade holder base 13 Handwhe...

Page 26: ...e for manual sectioning applications Smooth running hand wheel allows two manual sectioning modes rocking mode and conventional manual sectioning with full hand wheel rotation and 3 motorized sectioni...

Page 27: ...t distance without the need of deactivating the retraction Each change in the sense of rotation will be electronically detected and automatically converted into an advance or retraction movement of th...

Page 28: ...humidity 20 to max 80 non condensing Operating altitude Up to 2000 m above sea level Transport temperature range 29 C to 50 C Storage temperature range 5 C to 50 C Transport and storage relative humi...

Page 29: ...n orientation Maximum sectioning area with retraction 60 mm Maximum specimen block size for large standard clamp H x W x D Maximum specimen block size for super mega cassette clamp H x W x D 55 x 50 x...

Page 30: ...he instrument in rooms with an explosive hazard 4 2 Standard delivery packing list The HistoCore AUTOCUT is available in two configurations HistoCore AUTOCUT configuration 14 9AUTO00C1 Quantity Part d...

Page 31: ...ife holder set up and waste tray select one Specimen orientation 14050237717 Fine directional fixture for specimen clamp 14050238949 Directional fixture for specimen clamp 14050238160 Rigid fixture fo...

Page 32: ...trument Once unpacked the instrument must be moved only in an upright position Thoroughly follow the instructions given in the Unpacking Instructions attached at the outside of the package or the acti...

Page 33: ...ositioning Caution Accessories components loosening damaged during transportation Property damage or delayed diagnosis The packaging has two indicators ShockDot Impact Indicator and Tilt Indicator tha...

Page 34: ...uter carton wall Fig 13 7 Lift the instrument Fig 13 8 by holding it by the base plate at the front Fig 14 11 and under the instrument on the back Fig 14 12 and lift it out of the formed cushion Fig 1...

Page 35: ...and it may cause the delay of diagnosis Before assembling the handwheel check the feather key and make sure that it is in the handwheel shaft 2 Place the spacer Fig 15 4 and the handwheel Fig 15 5 on...

Page 36: ...ove the main power switch on the left rear side of the instrument The voltage setting is displayed in the viewing window Fig 16 1 1 Insert a small screwdriver into the cutout Fig 16 3 and carefully pr...

Page 37: ...1 3 4 If a foot switch is to be used with the instrument insert the foot switch cable Fig 18 1 into the connecting jack Fig 18 2 on the rear of the instrument and fasten it by tightening the screws Fi...

Page 38: ...nsporting the instrument wait at least two hours to allow the instrument to adopt the ambient temperature before turning it on Warning Misinterpretation of the yellow LED in the M STOP field Serious i...

Page 39: ...which setting was last activated This is indicated simultaneously on the control panel and microtome The LED of the active mode here the section thickness is illuminated in green When the red LED in t...

Page 40: ...e freeze Specimen damage and or delay of diagnose In case of a software freeze follow the instructions given in chapters Troubleshooting and Operation If instrument cannot set back into operation imme...

Page 41: ...tion thickness sum to 0 12 MENUMODE CLEAR Pressing two buttons simultaneously switches to the setting of the retraction value 5 1 2 Separate control panel Fig 21 1 9 5 3 11 7 2 10 6 4 12 8 13 17 15 19...

Page 42: ...MODE button Selects cutting mode 14 Button of setting sectioning window Sets sectioning window The green LED flashes until second sectioning window edge is set 15 MEMO button Sets one memory position...

Page 43: ...ion thickness setting range 0 50 m 100 m Setting values from 0 5 m 5 0 m in 0 5 m increments from 5 0 m 20 0 m in 1 0 m increments from 20 0 m 60 0 m in 5 0 m increments from 60 0 m 100 0 m in 10 0 m...

Page 44: ...coarse feed buttons have the same functions as in the trimming mode The STEP function is useful for careful step by step approximation of the specimen towards the knife How to activate the STEP functi...

Page 45: ...osition Fig 25 1 2 3 4 5 Set one memory position using the MEMO button Fig 25 1 on the separate control panel To set one memory position move the object head to the desired position and press the MEMO...

Page 46: ...ot turn the handwheel or press any button on the separate control panel or the instrument control panel during fast homing or moving to the memory position Caution The memory position is set too close...

Page 47: ...position of the object head In motorized sectioning mode the retraction depends on the setting of the sectioning speed In manual mode the retraction value can be selected in 5 m increments between 5 a...

Page 48: ...poses and does not indicate a particular speed Sectioning window setting Fig 30 1 The sectioning window function allows to optimally adapt the size of the sectioning area to the actual size of the spe...

Page 49: ...ing mode Turn the handwheel a short distance forwards and backwards for sectioning rocking mode Each change in the sense of rotation will be electronically detected and automatically converted into an...

Page 50: ...the electronic brake function does not work If the BRAKE button is pressed during manual sectioning a warning sound is heard Caution Using the electronic brake to lock the handwheel People injury Dam...

Page 51: ...ights up from the beginning of the remaining feed In addition an acoustic signal is heard for approx 2 seconds The sectioning process is interrupted and the object head stops in the upper end position...

Page 52: ...rating mode Press the foot switch once briefly to start motorized sectioning Press the foot switch again to stop it The specimen stops in the next upper end position Fig 45 SINGLE single stroke operat...

Page 53: ...d switch the instrument on while holding the slow forward coarse feed button Fig 49 1 on the separate control panel three digit display indicates C for clockwise Fig 49 2 To set counterclockwise rotat...

Page 54: ...t not be oriented during the retraction phase If a block is oriented during retraction the block will be fed by the retraction value plus the selected section thickness before the next section This ma...

Page 55: ...amp the specimen Set the object head to half the height of the vertical travel range by turning the handwheel Fig 51 If the object head remains in this exact position the setting is correct If the obj...

Page 56: ...bject head moves upwards turn the screw Fig 52 1 approx 1 2 turn counterclockwise each time Continue this procedure until the object head no longer moves once released Tip Repeat the adjusting procedu...

Page 57: ...he two in one blade holder E Fig 54 8 with the underside groove onto the T piece Fig 54 7 of the knife holder base Fig 53 2 3 To clamp retighten the eccentric bolt Fig 54 9 5 3 Adjusting the clearance...

Page 58: ...the specimen is clamped into place You can use the quick clamping system Fig 56 5 to hold all available accessory specimen clamps for more information see P 68 6 Optional Accessories To do so proceed...

Page 59: ...s provided in P 68 6 Optional Accessories 5 6 Clamping the knife disposable blade Warning Knives or blades are handled and or disposed in an inappropriate manner Serious injury of persons due to conta...

Page 60: ...ig 60 1 When using low profile blades the insert Fig 60 1 for low profile blades must first be placed in the two in one blade holder E and make sure that the bottom edge of the insert fits closely to...

Page 61: ...ser Fig 63 Fig 63 Warning Knives or blades are handled and or disposed in an inappropriate manner Serious injury of persons due to contact with extremely sharp knives and or blades Work especially tho...

Page 62: ...TRIM mode Set the desired trimming thickness Release the handwheel locking mechanisim and the handwheel brake lever In TRIM mode use the coarse feed buttons or electronic coarse feed wheel to move th...

Page 63: ...the handle of the handwheel Warning Inappropriate selection of sectioning speed Specimen damage Always set the sectioning speed according to the hardness of the specimen For hard specimens always sele...

Page 64: ...mask and safety goggles Warning Working with the instrument and brittle specimen without appropriate protective clothes Serious injury of persons by splinters while cutting brittle specimen Always wea...

Page 65: ...f trimming with the electronic coarse feed remember to switch back to sectioning mode Before starting sectioning always make sure that an appropriate sectioning thickness has been selected Fig 66 Alwa...

Page 66: ...men clamp prior to changing the specimen and during all work breaks Warning Uploading or unloading the specimen on the microtome without appropriate clothes and in an inappropriate manner Operator can...

Page 67: ...g knife Warning Paraffin waste falls on the floor and is not cleaned up Serious injury of persons for example by slipping and stumbling into the knife blade Always clean up paraffin waste before it sp...

Page 68: ...r specimen clamps Before assembling the fixture for specimen clamps activate the handwheel locking mechanism 6 1 1 Rigid fixture for specimen clamps Tips Remove the rubber ring Fig 68 5 only after att...

Page 69: ...the bores as shown and screw them in using an Allen key No 3 Insert the spring Fig 69 3 and pin Fig 69 4 with the flatter side into the thrust piece Fig 69 2 Completely screw in the thrust piece with...

Page 70: ...rectional fixture for specimen clamps with zero point indicators or the directional fixture for specimen clamps Screw the 4 screws Fig 71 2 into bore Fig 71 1 with an Allen key size 2 5 and tighten th...

Page 71: ...ble jaw upward against the fixed jaw to securely clamp the sample Warning Insufficient frame conditions for sectioning Specimen damage or poor sectioning results for example sections alternating in th...

Page 72: ...e V insert upward against the fixed jaw to securely clamp the sample 6 2 3 Foil clamp type 1 The foil clamp type 1 is appropriate both for clamping very small and thin foil pieces and flat angular spe...

Page 73: ...the standard specimen clamp as shown Turn the knurled screw Fig 75 6 clockwise to clamp the foil clamp in the standard specimen clamp 6 2 4 Universal cassette clamp Warning Insufficient frame conditi...

Page 74: ...cessary the specimen must be clamped horizontally Caution When using thin walled cassettes the cassettes become deformed or insecurely clamped into place or other problems because of the clamping syst...

Page 75: ...he left into the dovetail guide of the rigid fixture for specimen clamps and tighten screw Fig 77 1 Caution When using super mega cassette clamp the orientation is not set to position 0 when direction...

Page 76: ...9 To orient the inserted specimen insert the pin Fig 78 7 into the bore Fig 78 8 and rotate it counterclockwise to release the clamp You can now rotate the specimen so that the side you want faces upw...

Page 77: ...kwise 6 3 2 Knife holder E TC Warning The knife blade is installed before the knife blade holder and the knife blade holder base are installed on the instrument Serious injury of persons due to contac...

Page 78: ...low profile disposable blades The two in one blade holder E has a lateral displacement function so that the entire width of the blade can be used It is equipped with a blade ejector to safely remove t...

Page 79: ...otate the lever Fig 83 1 clockwise 6 3 4 Knife holder E with a water trough for low profile blades Fig 84 3 1 2 The knife holder E with a water trough Fig 84 is only for low profile blades The safety...

Page 80: ...ide knives profile c and d up to 16 cm long The integrated height adjustment feature allows you to also use knives that have been resharpened numerous times Knife holder N Fig 86 For holding conventio...

Page 81: ...ig 88 1 can be inserted whereas the shining facet of profile d knife Fig 88 2 should face the operator Putting the knife wrongly will damage the specimen and the knife Knife height adjustment When adj...

Page 82: ...s ease of cleaning due to the anti static surface Push the section waste tray Fig 89 1 from the front to the microtome base plate Fig 89 2 until it is held in place by the two magnets Fig 89 3 on the...

Page 83: ...acklighting illumination must be detached NEVER use the super mega cassette clamp with backlighting illumination The force balance system has to be adjusted when using the super mega cassette clamp 6...

Page 84: ...5 Fig 93 4 Support plate large for BIOCUT MULTICUT and AUTOCUT Fig 93 5 Support plate small for NANOCUT Fig 93 6 Allen key No 3 Fig 93 7 4 countersunk screws to install support plate Fig 93 8 Allen ke...

Page 85: ...n Fig 96 2 onto the baseplate from above and tighten with the Allen key size 8 Warning The universal microscope carrier tips Serious injury of persons After installing the vertical column immediately...

Page 86: ...oscope magnifying lens or cold light source please see the appropriate Instructions for Use 6 8 Magnifying lens LED illumination Tips The magnifying lens provides a 2x magnification and can be used wi...

Page 87: ...adjust the brightness of the spot lights Fig 99 5 1 6 7 2 3 4 8 9 Adjust the position of the magnifying lens to the specimen being processed The magnifying lens can be swiveled completely to the side...

Page 88: ...ventional knives up to 16 cm in length Height adjustment of the knife Separate clearance angle adjustment Movable safety guard Order No 14 0502 37993 Knife holder NZ silver Fig 102 For holding convent...

Page 89: ...3 click stop positions make it easier to utilize the entire blade length Color coded foldaway safety guard Order No 14 0502 38961 Knife holder E TC Fig 104 For tungsten carbide disposable blades TC 65...

Page 90: ...e it easier to utilize the entire blade length The pressure plate is replaceable Color coded foldaway safety guard With blade ejector Order No 14 0502 54497 Disposable blades low profile 819 Fig 107 8...

Page 91: ...rbide metal guarantees sections to approx 2 m The blades are fully recyclable Length 65 mm Thickness 1 mm Height 11 mm 1 pack of 5 pcs Order No 14 0216 26379 Knife 16 cm profile c steel Knife 16 cm pr...

Page 92: ...Order No 14 0216 04206 Fig 112 1 is the carbide metal knife serial number Knife case Fig 113 Variable knife case plastic for 1 or 2 knives 10 16 cm long Order No 14 0213 11140 Universal cassette clam...

Page 93: ...e directional Fig 117 Silver for holding the standard clamp universal cassette clamp round specimen holder and EM specimen holder including installation hardware Note Quick clamping system 14 0502 377...

Page 94: ...18 must be ordered separately Order No 14 0502 37717 Quick clamping system Fig 120 For specimen holder for use with the fine directional fixture for specimen clamps with zero point indicators 14 0502...

Page 95: ...ecimen clamps 14 0502 38005 and 14 0502 37998 black Maximum specimen size 25 x 13 mm Order No 14 0402 09307 EM specimen holder fixture Fig 124 For EM specimen holder black For samples with a 10 mm dia...

Page 96: ...er mega cassette clamp Fig 127 With adapter silver Note Use only together with non orientable specimen holder fixture 14 0502 38160 which has to be ordered separately Backlighting 14 0502 38719 cannot...

Page 97: ...men clamps to be changed quickly and reliably without modifying the handwheel Antistatic material makes cleaning easier Can be retrofitted for all rotary microtomes from HistoCore series Technical dat...

Page 98: ...nification Note Magnifier includes an adapter for holding the optional LED illumination Order No 14 0502 42790 Universal microscope carrier Fig 130 Universal assembly Order No 14 0502 40580 Backlighti...

Page 99: ...series Order No 14 0517 56261 Section waste tray Fig 133 For HistoCore rotary microtome series Anti static section waste tray Order No 14 0517 56237 Section waste tray Order No 14 0518 56458 Brush Fig...

Page 100: ...high profile 1 pack of 50 80 x 14 x 0 35 mm 14 0358 38926 Disposable blades high profile 10 packs of 50 14 0358 38383 Disposable tungsten carbide blades TC 65 14 0216 26379 Knife 16 cm profile c stee...

Page 101: ...unction with LED 1000 control unit Order No 14 6000 04825 14 6000 04826 LED 1000 control unit This module can only function with LED 1000 Hi power spots 2 arm Order No 14 6000 04826 14 6000 04825 Back...

Page 102: ...instrument and check if error code E2 still exists 2 If error code E2 still exists call Leica Biosystems service Error code E3 is displayed a two second long warning beep is heard The instrument does...

Page 103: ...amped in the universal cassette clamp If the universal cassette clamp is soiled by paraffin clean the universal cassette clamp P 109 8 1 Cleaning the instrument When using cassettes whose lid is molde...

Page 104: ...mped into place an alternative tensioning clamp must be used Laterally displace the knife blade holder or insert a new knife blade Insert a new pressure plate or use a new two in one blade holder Read...

Page 105: ...ed and or section thickness when trimming Select a smaller section thickness rotate the handwheel more slowly Adjust the clearance angle 7 3 Instrument malfunctions The following is a list of the most...

Page 106: ...ment was switched on Select the trimming mode by pressing TRIM SECT Then retract the specimen using the coarse feed button See above The coarse feed buttons can be used to move the object in a backwar...

Page 107: ...the clamping lever counterclockwise Fig 137 2 3 Pull out the bolt Fig 137 3 Fig 138 3 4 Remove the damaged pressure plate Fig 138 4 Tips Make sure that the insert of the clamping top is installed as...

Page 108: ...a 2 5 mm Allen key at the setscrew Fig 139 1 so that the lever initially slips when the lever is activated Continue turning the setscrew Fig 139 1 a little bit approx 1 4 turn to the left or right th...

Page 109: ...of persons due to contact with extremely sharp knife blade Before uninstalling the knife blade holder from the microtome always make sure to detach the knife blade wearing cut resistant gloves and st...

Page 110: ...and activate the handwheel lock Switch the instrument off and unplug it Remove the blade from the blade holder and insert it in the receptacle at the bottom of the dispenser or remove the knife from t...

Page 111: ...141 7 and take the pressure plate off 3 Remove the clamping top To do so rotate the clamping lever Fig 141 2 of the lateral displacement counterclockwise and pull it out sideways push the clamping top...

Page 112: ...e of the clamping top Fig 141 3 Tips If the insert of the clamping top is installed incorrectly the pressure plate cannot be clamped Universal cassette clamp Fig 142 1 2 3 Detach cassette clamp Fig 14...

Page 113: ...e internal components of the instrument for service and repair The instrument is basically maintenance free To ensure trouble free operation of the instrument over a long period of time the following...

Page 114: ...tems service technician regardless of how heavily the instrument has been used 8 4 Lubricating the instrument Once a month lubricate the following parts with the included drive parts oil 1 2 drops are...

Page 115: ...e holder E Fig 146 1 2 Clamping lever Fig 146 1 for the lateral displacement Clamping lever Fig 146 2 for the blade Universal cassette clamp Fig 147 1 2 Lubricate the spiral springs Fig 147 1 of the t...

Page 116: ...ty terms of your Leica Biosystems sales organization or the organization from which you have purchased the contractual product shall apply exclusively 9 2 Service information If you are in need of tec...

Page 117: ...LeicaBiosystems com within the product menu This template has to be used for gathering all required data When returning a product a copy of the filled and signed confirmation has to be enclosed or pa...

Page 118: ...118 Version 1 1 Revision B Notes...

Page 119: ......

Page 120: ...w LeicaBiosystems com Version 1 1 Revision B 10 2018 Leica Biosystems Nussloch GmbH Heidelberger Str 17 19 D 69226 Nussloch Germany Tel 49 0 6224 143 0 Fax 49 0 6224 143 268 Web www LeicaBiosystems co...

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