Biotek Synergy HTX, Operator'S Manual

Page: 157 / 184

Dispense Module Tests |
135
d.
Remove the plate and weigh it. Record the weight and tare the balance.
e.
Place the plate on the carrier, and dispense 5 µL/well to columns 9–12.
f.
Remove the plate and weigh it. Record the weight.
g.
Manually pipette 150 µL of deionized or distilled water into all 12 columns,
on top of the green test dye solution.
h.
Place the plate on the carrier for a 15-second shake, the “80 µL” read at
405/750 nm, and the “20 and 5 µL” read at 630/750 nm.
7.
When processing is complete, select
File > Save As
. Enter an identifying file
name and click
Save
.
8.
Remove the plate from the carrier and set it aside.
9.
Repeat steps 4–9 using
Synergy HTX Disp 2 Test.prt
.
10.
See
Results Analysis
on page 137 for instructions on analyzing the results.

When all tests are complete, prime both dispensers with at least
5000 µL of deionized water to flush out the green dye solution.
Procedure for Models without Absorbance Capabilities
If you will not be using a BioTek absorbance reader for this procedure, prepare your
reader to perform two reads with the following characteristics:
80 µL Read 20 & 5 µL Read
Primary Wavelength: 405 nm 630 nm
Reference Wavelength: 750 nm 750 nm
Plate Columns:
1
–
4 5
–
12
1.
If you have not already done so, create the necessary Gen5 protocols as
described on page 138.
2.
Prime both dispensers with 4000 µL of deionized or distilled water.
3.
Remove the inlet tubes from the supply bottles. Prime both dispensers with the
Volume set to 2000 µL. This prevents the water from diluting the dye.
4.
Fill a beaker with at least 20 mL of the green dye solution. Prime both
dispensers with 2000 µL of the solution. When finished, remove the priming
plate from the carrier.
5.
In Gen5, create an experiment based on the
Synergy HTX Disp 1 Test No
Read
protocol.
6.
Place a new 96-well microplate on the balance and tare the balance.
7.
Place the plate on the microplate carrier.
Synergy HTX Operator's Manual