Biotek Synergy HTX, Operator'S Manual

Page: 67 / 184

Gen5 Software |
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• Add a Read step to specify the detection method and filter sets or
wavelength values, enable time-resolved fluorescence, and set the Top
Probe Vertical Offset value.
• To define a Kinetic read, place an Endpoint Read step inside a Kinetic
Start/End loop.
5.
Open the Plate Layout dialog and assign blanks, samples, controls, and/or
standards to the plate.
6.
Open the Data Reduction dialog to add data reduction steps. Categories
include Transformation, Well Analysis, Curve Analysis, and more.
7.
Create a report or export template, via one of the Report/Export Builder
options.
8.
Save the file with an identifying name.
The instructions below briefly describe how to create an Experiment based on an
existing protocol and then read a plate. See Gen5’s Help system for complete
instructions.
1.
To create a new experiment, from the Task Manager click,
Experiments >
Create using an existing protocol
.
2.
Select the desired protocol and click
OK
.
3.
Select
Plate > Read
or click the Read Plate icon.
4.
Click
OK
when the Load Plate dialog appears. The plate will be read.
5.
When the read is complete, measurement values will appear in Gen5. To
view them, select the desired data set (e.g., “528/20,645/40”) from the Data
drop-down list.
6.
If you have not already done so, save the file with an identifying name.
Controlling the Dispense Module
Applies only to Synergy HTX models with injectors.
Gen5 is used to perform several dispense module-specific functions, including
initializing, priming, and purging. Gen5 also contains certain configuration items that
must be set before using the dispense module. Read the following sections to become
familiar with these functions and configuration items.
Initialization
If the dispense module was connected to the reader before the reader was turned
on, or if a System Test was run via Gen5, the dispense module should initialize
automatically. If for any reason the module does not initialize automatically, you
can initialize it from Gen5:
1.
In Gen5, select
System > Instrument Control > Synergy HTX
and click
the
Prime
tab.
Synergy HTX Operator's Manual