Biotek Synergy HTX, Operator'S Manual

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Flush/Purge the Fluid Path |
57
Flush/Purge the Fluid Path
Applies only to Synergy HTX models with injectors.
At the end of each day that the dispense module is in use, flush the fluid path using Gen5’s
priming utility. Leave the fluid to soak overnight or over a weekend, and then purge the
fluid before using the instrument again.

This flushing and purging routine is also recommended before
disconnecting the outlet tubes from the rear of the reader, and before
decontamination to remove any assay residue prior to applying
isopropyl alcohol or sodium hypochlorite.
To flush the fluid path:
1.
Fill two supply bottles with deionized or distilled water. Insert the supply
(inlet) tubes into the bottles.
2.
Place the priming plate on the carrier.
3.
From Gen5’s main screen, select
System > Instrument Control.
Select the
appropriate reader if prompted.
4.
Click the
Prime
tab and select
Dispenser 1 .
5.
Set the
Volume
to 5000 µL. Keep the default prime
Rate
.
6.
Click
Prime
to start the process. When the process is complete, carefully
remove the priming plate from the carrier and empty it.
7.
Repeat the process for
Dispenser 2 .
Leave the water in the system overnight or until the instrument will be used
again. Purge the fluid from the system (see below) and then prime with the
dispense reagent before running an assay.
To purge the fluid from the system:
1.
Place the inlet tubes in empty supply bottles or a beaker.
2.
Select
System > Instrument Control
. Select the appropriate reader if
prompted.
3.
Click the
Prime
tab and select
Dispenser 1
.
4.
Set the
Volume
to 2000 µL.
5.
Click
Purge
to start the process.
When the purge is complete, repeat the process for
Dispenser 2
.

After purging the system, you may wish to run a quick Dispense
protocol to visually verify the dispense accuracy. See the next page
for instructions for creating the protocol.
Synergy HTX Operator's Manual