Biotek Synergy HTX, Operator'S Manual

Page: 151 / 184

Fluorescence Tests |
129
Pipette Map

Seal the plate with foil or store it in a black polyethylene bag until use.
Perform these steps carefully and refer to the grid that follows.
For the
Sensitivity
test (dark gray wells):
• Pipette 150 µL of CBB buffer into wells C9, D9, and E9.
• Pipette 150 µL of the
17.6 ng/mL
(100 nM) MUB solution into well D7.
For the
Linearity
test (wells C1-F5):
• Use a multi-channel pipette with just four tips installed.
•
Pipette 150 µL of buffer solution into wells C2-F5. Discard the tips.
•
Pipette 150 µL of the
17.6 ng/mL
(100 nM) solution into wells C1-F1.
• Pipette 150 µL of the 17.6 ng/mL (100 nM) solution into wells C2-F2.
Mix the wells using the pipette. Do not discard the tips.
•
Aspirate 150 µL from wells C2-F2 and dispense into wells C3-F3.
Mix the wells using the pipette. Do not discard the tips.
•
Aspirate 150 µL from wells C3-F3 and dispense into wells C4-F4.
Mix the wells using the pipette. Do not discard the tips.
• Aspirate 150 µL from wells C4-F4 and dispense into wells C5-F5.
Mix the wells using the pipette.
• Aspirate 150 µL from wells C5-F5 and discard.
Synergy HTX Operator's Manual